Nucleic Acid Extraction Kit (A01)
Kuona musimboti
Mushure mekuburitsa genomic DNA nekutsemura maseru ane lysis buffer, iyo magineti bead inogona kusarudza kusungira kune genomic DNA mumuenzaniso.Nhamba shoma yetsvina inotorwa nemagnetic bead inogona kubviswa newash buffer.MuTE, iyo magineti bead inogona kuburitsa iyo boundgenome DNA, ichiwana yemhando yepamusoro genome DNA.Iyi nzira iri nyore uye inokurumidza uye yakabviswa DNA mhando yakakwirira, iyo inogona kusangana nezvinodiwa zvekuonekwa kweDNA methylation.Zvichakadaro, kit yekubvisa yakavakirwa pane magineti bead inogona kuenderana neotomatiki nucleic acid kudhirowa, kusangana nepamusoro-kuburikidza nucleic acid yekubvisa mabasa.
Zvikamu zvikuru zve reagent
Izvo zvikamu zvinoratidzwa mutafura 1:
Tafura 1 Reagent Zvikamu uye Kurodha
| Zita rechikamu | Main components | Saizi (48) | Saizi (200) |
| 1. Lysis mhinduro | Guanidine hydrochloride, Tris | 11 mL / bhodhoro | 44 mL / bhodhoro |
| 2. Zvigadziriso zvekuchenesa A | NaCl, Tris | 11 mL / bhodhoro | 44 mL / bhodhoro |
| 3. Mishonga yekuchenesa B | NaCl, Tris | 13 mL / bhodhoro | 26.5mL/bhodhoro *2 |
| 4. Eluent | Tris, EDTA | 12 mL / bhodhoro | 44 mL / bhodhoro |
| 5. Protease K solution | Protease K | 1.1mL/chidimbu | 4.4mL/chidimbu |
| 6. Magnetic bead kuturika 1 | Magnetic beads | 1.1mL/chidimbu | 4.4mL/chidimbu |
| 7. Mirayiridzo yekutora nucleic acid reagents |
| 1 kopi | 1 kopi |
Zvikamu zvinodikanwa mune nucleic acid kubviswa, asi hazvina kuisirwa mukiti:
1. Reagent: Anhydrous ethanol, isopropanol uye PBS;
2. Zvishandiso: 50ml centrifuge tube uye1.5ml EP tube;
3. Equipment: Mvura yekugezera kettle, pipettor, magnetic shelf, centrifuge, 96-gomba yakadzika ndiro (otomatiki), automatic nucleic acid extraction equipment (otomatiki).
Basic information
Sample zvinodiwa
1. Kusvibiswa kwemuchinjikwa pakati pemasampuli kunofanira kudziviswa mumuenzaniso wekuunganidza uye kuchengetedza.
2.Kuonekwa kuchapedzwa pasi pekuchengetedza kwemazuva 7 kwekushisa kwemukati mushure mekuunganidza kwekervical exfoliated cell sample (isina-fixed).Kuonekwa kunopedzwa pasi pekuchengetwa kwemazuva makumi matatu kwekushisa kwemukati mushure mekuunganidzwa kwemuenzaniso weti;Kuonekwa kunopedzwa nenguva mushure mekuunganidzwa kwemasero akagadzirwa.
Madiro ekupaka:200 pcs / bhokisi, 48 pcs / bhokisi.
Mamiriro ekuchengetera:2-30 ℃
Nguva yechokwadi:12 mwedzi
Chishandiso chinoshanda:Tianlong NP968-C nucleic acid extraction instrument, Tiangen TGuide S96 nucleic acid extraction instrument, GENE DIAN EB-1000 nucleic acid extraction instrument.
Medical mudziyo rekodhi chitupa Nha./chigadzirwa tekinoroji chinodiwa Nha.: HJXB No. 20210099.
Zuva rekubvumirwa uye kugadziriswa kwemirairo:
Zuva remvumo: Mbudzi 18, 2021
