Nucleic Acid Extraction Kit (A02)
Kuona musimboti
Mushure mekuburitsa genomic DNA nekutsemura maseru ane lysis buffer, iyo magineti bead inogona kusarudza kusungira kune genomic DNA mumuenzaniso.Nhamba shoma yetsvina inotorwa nemagnetic bead inogona kubviswa newash buffer.MuTE, iyo magineti bead inogona kuburitsa iyo boundgenome DNA, ichiwana yemhando yepamusoro genome DNA.Iyi nzira iri nyore uye inokurumidza uye yakabviswa DNA mhando yakakwirira, iyo inogona kusangana nezvinodiwa zvekuonekwa kweDNA methylation.Zvichakadaro, kit yekubvisa yakavakirwa pane magineti bead inogona kuenderana neotomatiki nucleic acid kudhirowa, kusangana nepamusoro-kuburikidza nucleic acid yekubvisa mabasa.
Zvikamu zvikuru zve reagent
Izvo zvikamu zvinoratidzwa mutafura 1:
Tafura 1 Reagent Zvikamu uye Kurodha
| Zita rechikamu | Main components | Saizi (48) | Saizi (200) |
| 1. Digestion Buffer A | Tris, SDS | 15.8 mL / bhodhoro | 66mL/bhodhoro |
| 2. Lysis Buffer L | Guanidinium Isothiocyanate, Tris | 15.8 mL / bhodhoro | 66mL/bhodhoro |
| 3. Wash Buffer A | NaCl, Tris | 11 mL / bhodhoro | 44 mL / bhodhoro |
| 4. Wash Buffer B | NaCl, Tris | 13 mL / bhodhoro | 26.5mL/bhodhoro *2 |
| 5. TE | Tris, EDTA | 12 mL / bhodhoro | 44 mL / bhodhoro |
| 6. Protease K solution | Protease K | 1.1mL/chidimbu | 4.4mL/chidimbu |
| 7. Magnetic bead kuturika 2 | Magnetic beads | 0.5mL / chidimbu | 2.2mL / chidimbu |
| 8. Mirayiridzo yekutora nucleic acid reagents | / | 1 kopi | 1 kopi |
Zvikamu zvinodikanwa mune nucleic acid kubviswa, asi hazvina kuisirwa mukiti:
1. Reagent: Anhydrous ethanol, isopropanol, uye PBS;
2. Zvishandiso: 50mL centrifuge tube uye1.5mL EP tube;
3. Equipment: Mvura yekugezera, pipettes, magnetic shelf, centrifuge, 96-tsime ndiro (otomatiki), automatic nucleic acid extraction equipment (otomatiki).
Basic information
Sample zvinodiwa:
1.Kuonekwa kuchapedzwa pasi pekuchengetedza kwemazuva 7 kwekushisa kwemukati mushure mekuunganidza kwekervical exfoliated cell sample (isina-fixed).
2.Kuonekwa kuchapedzwa pasi pekuchengetedza kwemazuva makumi matatu ekupisa kwemamiriro ekunze mushure mekuunganidzwa kwecervical exfoliated cell sample (yakagadziriswa).
3.Kuonekwa kuchapedzwa pasi pekuchengetwa kwemazuva makumi matatu kwekushisa kwemukati mushure mekuunganidzwa kwemuenzaniso weti;Kuonekwa kunopedzwa nenguva mushure mekuunganidzwa kwemasero akagadzirwa.
Madiro ekupaka:200 pcs / bhokisi, 48 pcs / bhokisi.
Mamiriro ekuchengetera:2-30 ℃
Nguva yechokwadi:12 mwedzi
Chishandiso chinoshanda:Tianlong NP968-C nucleic acid extraction instrument, Tiangen TGuide S96 nucleic acid extraction instrument, GENE DIAN EB-1000 nucleic acid extraction instrument.
Medical mudziyo rekodhi chitupa Nha./chigadzirwa tekinoroji chinodiwa Nha.:HJXB No. 20210100.
Zuva rekubvumirwa uye kudzokororwa kwemirairo:Zuva remvumo: Mbudzi 18, 2021
Nezvedu
Sebhizimusi repamusoro-soro rakavambwa muna 2018 nenyanzvi dzepamusoro epigenetic, Epiprobe inotarisa pakuongororwa kwema molecular cancer DNA methylation uye indasitiri yekurapa chaiyo.Tine hwaro hwakadzama hwetekinoroji, isu tinovavarira kutungamira nguva yezvigadzirwa zvitsva kunyudza cancer mubud!
Zvichienderana neEpiprobe musimboti wechikwata chekutsvaga kwenguva refu, kusimudzira uye shanduko mumunda weDNA methylation neinocheka-kumucheto hunyanzvi, yakasanganiswa neyakasarudzika DNA methylation zvinangwa zvegomarara, isu tinoshandisa yakasarudzika multivariate algorithm inosanganisa hombe data uye yekugadzira njere tekinoroji. vakazvimiririra gadzira yakasarudzika patent-yakachengetedzwa mvura biopsy tekinoroji.Nekuongorora iyo methylation level yenzvimbo dzakanangana dzemahara eDNA zvimedu mumuenzaniso, kukanganisa kwemaitiro echinyakare nzira dzekuongorora uye miganhu yekuvhiya uye puncture sampling inodziviswa, izvo zvisingaite chete kuwana kunyatsoonekwa kwekenza yekutanga, asi zvakare inogonesa chaiyo-nguva yekutarisa. yekenza kuitika uye kukura dynamics.
