TAGMe DNA Methylation Detection Kits(qPCR) yeCervical Cancer
PRODUCT FEATURES
Precision
Yakasimbiswa pamusoro pe36000 kliniki samples mune kaviri-mapofu akawanda-epakati zvidzidzo, chigadzirwa chine chaiyo ye94.3% uye kunzwa kwe96.0%.
Convenient
Iyo yekutanga Me-qPCR methylation yekuona tekinoroji inogona kupedzwa mudanho rimwe mukati meawa matatu pasina bisulfite shanduko.
Kurumidza
Kuongororwa gomarara remuchibereko kunogona kukwidziridzwa kusvika padanho repamusoro-soro (precancerous lesions) nhanho.
Automation
Inoperekedzwa neyakagadzirirwa mhedzisiro yekuongorora software, dudziro yemhedzisiro ndeye otomatiki uye inoverengwa zvakananga.
ZVIRI KUSHANDISA
Iyi kit inoshandiswa mu in vitro qualitative yekuona hypermethylation yejini PCDHGB7 mune zvienzaniso zvekervical.Mhedzisiro yakanaka inoratidza kuwedzera kwengozi yegiredhi 2 kana yepamusoro-giredhi / yakawedzera yepamusoro cervical intraepithelial neoplasia (CIN2 +, kusanganisira CIN2, CIN3, adenocarcinoma in situ, uye kenza yechibereko), izvo zvinoda imwe colposcopy uye / kana histopathological kuongorora.Pane zvinopesana, mhinduro dzisina kunaka dzebvunzo dzinoratidza kuti njodzi yeCIN2 + yakaderera, asi njodzi haigone kubviswa zvachose.Kuongororwa kwekupedzisira kunofanirwa kuve kwakavakirwa pacolposcopy uye / kana histopathological mhedzisiro.PCDHGB7 inhengo yemhuri yeprotocadherin γ gene cluster.Protocadherin yakawanikwa inodzora zvipenyu zvakaita sekuwedzera kwesero, kutenderera kwesero, apoptosis, kupinda, kutama uye autophagy yemasero ebundu kuburikidza nenzira dzakasiyana-siyana dzekusaina, uye kunyarara kwemajini kunokonzerwa ne hypermethylation yedunhu rinosimudzira zvine hukama zvakanyanya nekuitika uye kusimudzira. kenza dzakawanda.Zvinonzi hypermethylation yePCDHGB7 inosanganiswa nemhando dzakasiyana-siyana dzemamota, akadai seasiri Hodgkin lymphoma, kenza yemazamu, kenza yechibereko, endometrial cancer uye dundira.
ZVINHU ZVINOTAURWA
Iyi kit ine nucleic acid yekubvisa reagent uye PCR yekuona reagent.Nucleic acid inobviswa nemagnetic-bead-based method.Iyi kit yakavakirwa pamusimboti wefluorescence quantitative PCR nzira, uchishandisa methylation-specific real-time PCR reaction yekuongorora template DNA, uye panguva imwe chete kuona CpG nzvimbo dzePCDHGB7 gene uye yemhando yekudzora marker yemukati referensi gene zvidimbu G1 uye G2.Iyo methylation level yePCDHGB7 mumuenzaniso, kana iyo Me value, inoverengerwa maererano nePCDHGB7 gene methylated DNA amplification Ct kukosha uye Ct kukosha kwereferensi.Iyo PCDHGB7 gene hypermethylation yakanaka kana isina kunaka chimiro inotarwa maererano neMe value.
Maitiro ekushandisa
Kuongororwa kwekutanga
Vanhu vane hutano
Cancer Risk Assessment
Huwandu hwenjodzi yepamusoro (yakanaka kune yakanyanya-njodzi yepillomavirus yemunhu (hrHPV) kana yakanaka kune cervical exfoliation cytology)
Recurrence Monitoring
Postoperative vanhu (vane nhoroondo yemhando yepamusoro-giredhi yecervical maronda kana kenza yechibereko)
Clinical kukosha
Kuongororwa kwekutanga kwehuwandu hwevanhu vane hutano:Gomarara remuchibereko nemaronda emudumbu anogona kunyatsoongororwa
Kuongororwa kwenjodzi muhuwandu hwehuwandu hwenjodzi:Kurongeka kwenjodzi kunogona kuitwa muHPV-positive vanhu kutungamira kunotevera kuongororwa
Recurrence monitoring ye postoperative population:Postoperative recurrence monitoring inogona kuitwa kudzivirira kunonoka kurapwa kunokonzerwa nekudzokorora
Sample collection
Sampling nzira: Isa cervical sampler inoraswa pacervical os, zvinyoro nyoro puruzira cervical brashi uye tenderedza 4-5times wachi, bvisa zvishoma nezvishoma cervical brashi, woiisa mucell preservation solution, woinyora pakuongorora kunotevera.
Kuchengetedza sampuli:Masamples anogona kuchengetwa pakamuri tembiricha anosvika mazuva gumi nemana, pa2-8 ℃ kweanosvika mwedzi miviri, uye pa -20±5℃ kusvika kumwedzi makumi maviri nemana.
Maitiro ekuona: Maawa matatu (Pasina manyorero maitiro)
DNA Methylation Detection Kits (qPCR) yeUrothelial Cancer
| Clinical application | Clinical auxiliary diagnosis yegomarara rechibereko |
| Detection gene | PCDHGB7 |
| Muenzaniso mhando | Mienzaniso yekervical yevakadzi |
| Test method | Fluorescence quantitative PCR tekinoroji |
| Inoshanda modhi | ABI7500 |
| Packing specification | 48 bvunzo/kiti |
| Storage Conditions | Kit A inofanira kuchengetwa pa2-30 ℃ Kit B inofanira kuchengetwa pa -20±5℃ Inoshanda kusvika kumwedzi gumi nemiviri |
